Screening method for pain suppressor and pharmaceutical composition for prevention or treatment of pain
AssigneeOsaka University
InventorsToshihide Yamashita, Yasufumi Hayano, Moe Yamada
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Abstract
The present invention provides a screening method for a pain suppressor, which method comprises using FLRT3 to select a substance capable of inhibiting FLRT3 expression or a substance capable of inhibiting FLRT3 transport to the spinal cord. In addition, the present invention provides a pharmaceutical composition for prevention or treatment of pain, which pharmaceutical composition comprises, as an active ingredient, a substance capable of inhibiting FLRT3 expression or a substance capable of inhibiting FLRT3 transport to the spinal cord.
Claims13
A screening method for a pain suppressor substance, the method comprising:
- (a) contacting cells that express FLRT3 with a test substance;
- (b) measuring the level of FLRT3 expression in the cells that express FLRT3 after (a);
- (c) comparing the level of FLRT3 expression measured in (b) to a control population of FLRT3-expressing cells, which were not contacted with the test substance;
- and
- (d) selecting a test substance as a pain suppressor substance when said test substance reduces the level of FLRT3 expression in said cells that express FLRT3 after contact with said test substance, as compared to said control population of FLRT3-expressing cells, which were not contacted with the test substance.
2-13. (canceled)
A screening method for a pain suppressor substance, the method comprising:
- bringing a test substance into contact with FLRT3 and Unc5B;
- assessing FLRT3 binding to Unc5B;
- and
- determining whether or not the test substance inhibits FLRT3 binding to Unc5B.
A method for the inhibition or treatment of pain in a subject, comprising:
- administering an effective amount of a pain suppressor substance to said subject, wherein said pain suppressor substance comprises a nucleic acid comprising a sequence that inhibits FLRT3 expression.
A method for the inhibition or treatment of pain in a subject, comprising:
- administering an effective amount of a pain suppressor substance to said subject, wherein said pain suppressor substance comprises a peptide comprising a sequence that inhibits an interaction between FLRT3 and Unc5B.
Dependencies are inferred from the claim text.