Immunological detection method and kit for mycoplasma pneumoniae

An immunochromatographic assay for detecting Mycoplasma pneumoniae, comprising:

• providing a membrane carrier having a capturing zone that is formed by previously immobilizing a first antibody against P30 protein of Mycoplasma pneumoniae at a predetermined position;
• chromatographically developing a liquid mixture in the membrane carrier toward the capturing zone, said liquid mixture containing a second antibody against the P30 protein and a predetermined amount of a test sample, whereby a complex of an antigen contained in the test sample and the second antibody is captured by the capturing zone,
• wherein at least one of the first and second antibodies is an antibody against an epitope of P30 protein located in an amino acid sequence of SEQ ID NO:
• 3 or 4.

A Mycoplasma pneumoniae-detecting immunochromatographic test strip, comprising at least first and second antibodies against P30 protein of Mycoplasma pneumoniae and a membrane carrier,

• wherein the first antibody is previously immobilized at a predetermined position of the membrane carrier so as to form a capturing zone;
• and
• the second antibody is labeled with an appropriate labeling substance and is provided at a position separated from the capturing zone so as to be chromatographically developed in the membrane carrier,
• wherein at least one of the first and second antibodies is an antibody against an epitope of P30 protein located in an amino acid sequence of SEQ ID NO:
• 3 or 4.