Novel insecticidal proteins toxic or inhibitory to hemipteran pests
AssigneeMonsanto Technology LLC
InventorsDavid J. Bowen, Catherine A. Chay, Arlene R. Howe, Jennifer L. Lutke
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Abstract
Nucleotide sequences are disclosed encoding novel, insecticidal TIC4747 and related proteins exhibiting Hemipteran and Lepidopteran inhibitory activity, as well as fragments thereof. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing a polynucleotide construct encoding one or more of the toxin proteins within the TIC4747-related protein toxin class.
Claims22
A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding a pesticidal protein or fragment thereof, wherein:
- a) said pesticidal protein comprises the amino acid sequence of SEQ ID NO:12, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, or SEQ ID NO:28;
- or
- b) said pesticidal protein comprises an amino acid sequence having at least 62% amino acid sequence identity to the amino acid sequence of SEQ ID NO:12, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, or SEQ ID NO:28;
- or
- c) said polynucleotide segment hybridizes under stringent hybridization conditions to a polynucleotide comprising the nucleotide sequence of SEQ ID NO:11, SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, or SEQ ID NO:27 under hybridization conditions of 68° C., followed by washing at 68° C., in 2×SSC containing 0.1% SDS.
A plant comprising a recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding a pesticidal protein or fragment thereof, wherein:
- a) said pesticidal protein comprises the amino acid sequence of SEQ ID NO:12, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, or SEQ ID NO:28;
- or
- b) said pesticidal protein comprises an amino acid sequence having at least 62% amino acid sequence identity to the amino acid sequence of SEQ ID NO:12, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, or SEQ ID NO:28;
- or
- c) said polynucleotide segment hybridizes under stringent hybridization conditions to a polynucleotide comprising the nucleotide sequence of SEQ ID NO:11, SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, or SEQ ID NO:27 under hybridization conditions of 68° C., followed by washing at 68° C., in 2×SSC containing 0.1% SDS;
- or
- d) said plant exhibits a detectable amount of said pesticidal protein, wherein the pesticidal protein comprises an amino acid sequence of SEQ ID NO:12, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, or SEQ ID NO:28.
A method for controlling a Lepidopteran species pest, and controlling a Hemipteran species infestation of a plant, said method comprising contacting the pest with an insecticidally effective amount of one or more pesticidal proteins comprising an amino acid sequence having at least 62% amino acid sequence identity to SEQ ID NO:12, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, or SEQ ID NO:28.
Dependencies are inferred from the claim text.