Methods and compositions for improving plant traits
AssigneePivot Bio, Inc.
InventorsKarsten TEMME, Alvin TAMSIR, Sarah BLOCH, Rosemary CLARK, Emily TUNG
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Abstract
Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.
Claims20
A method of increasing the amount of atmospheric derived nitrogen in a non-leguminous plant, comprising:
- exposing said non-leguminous plant to engineered non-intergeneric microbes, said engineered non-leguminous microbes comprising at least one genetic variation introduced into a nitrogen fixation genetic regulatory network or at least one genetic variation introduced into a nitrogen assimilation genetic regulatory network.
A method of increasing an amount of atmospheric derived nitrogen in a corn plant, comprising exposing said corn plant to engineered non-intergeneric microbes comprising engineered genetic variations within at least two genes selected from the group consisting of niJL, glnB, and amtB.
A method of increasing an amount of atmospheric derived nitrogen in a corn plant, comprising:
- exposing said corn plant to engineered non-intergeneric microbes comprising at least one genetic variation introduced into a nitrogen fixation genetic regulatory network and at least one genetic variation introduced into a nitrogen assimilation genetic regulatory network, wherein said engineered non-intergeneric microbes, in planta, produces at least 5% of fixed nitrogen in said corn plant as measured by dilution of 15N in crops grown in fields treated with fertilizer containing 1.2% 15N.
Dependencies are inferred from the claim text.