Nano-bioreactor apparatus and method of manipulating extracellular metabolic systems

An enzymatic nano-bioreactor apparatus, comprising:

• a plurality of input pumps configured to introduce at least one input to a plurality of micro-bioreactors connected each other by a connector, and
• at least one nano-bioreactor placed inside the micro-bioreactor, configured to receive the input, wherein the nano-bioreactor comprising:
• at least one enzyme, and
• a nano-fiber,
• wherein the enzyme is a nanoparticle configured to immobilize on the nano-fiber, and
• an outlet configured to extrude a resultant output from the nano-bioreactor to a collector unit.

A method for manipulating extracellular metabolic system, comprising:

• introducing an input via a plurality of input pumps to one or more micro-bioreactors connected each other by a connector;
• receiving the input by a first nano-bioreactor placed inside the first micro-bioreactor to produce first output;
• receiving the first output by a second nano-bioreactor placed inside the second micro-bioreactor to produce second output;
• receiving the second output by a third nano-bioreactor placed inside the third micro-bioreactor to produce final output, and
• extruding and collecting the final output via an outlet to a collector unit,
• wherein the plurality of micro-bioreactors are connected each other by a silicon connector,
• wherein the second nano-bioreactor comprises glutamate dehydrogenase enzyme nanoparticles immobilized on the nano-fiber to form a glutamate dehydrogenase nano-bioreactor, and the third nano-bioreactor comprises glutamine synthetase enzyme nanoparticles immobilized on the nano-fiber to form a glutamine synthetase nano-bioreactor.