Spin columns comprising poly(acid) membrane separation matrices, and methods of making and using the same
AssigneeTakara Bio USA, Inc.
InventorsGeorge G. Jokhadze, Sayantan Mitra
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Abstract
Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.
Claims30
A spin column comprising:
- an elongated hollow structure having a sample inlet at a first end and a sample outlet at a second end;
- and
- a poly(acid) membrane matrix positioned in the elongated hollow structure such that fluid must flow through the poly(acid) membrane to traverse the structure from the first end to the second end.
The spin column according to any of the preceding claims, wherein the elongated hollow structure is a tube.
The spin column according to any of the preceding claims, wherein the poly(acid) membrane is positioned proximal to the second end.
The spin column according to any of the preceding claims, wherein the poly(acid) matrix comprises two or more stacked membranes each having a poly(acid) component adsorbed to a surface of a porous membrane support.
The spin column according to any of the preceding claims, wherein the poly(acid) matrix comprises three or more stacked membranes each having a poly(acid) component adsorbed to a surface of a porous membrane support.
The spin column according to any of the preceding claims, wherein the sample inlet has a first diameter and the sample outlet has a second diameter.
The spin column according to any of the preceding claims, wherein the column has a volume ranging from 1 μl to 1 liter.
The spin column according to any of the preceding claims, wherein the spin column comprises a frit in supporting relationship to the poly(acid) membrane.
The spin column according to any of the preceding claims, wherein the spin column is nested in a collection tube.
The spin column according to any of the preceding claims, wherein the spin column comprises a cap at the first end.
Dependencies are inferred from the claim text.