Device for solid phase extraction and method for use thereof
AssigneeWaters Technologies Corporation
InventorsPamela C. Iraneta, Xin Zhang, Frank John Marszalkowski, Jr.
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Abstract
Disclosed is a device for a solid phase extraction comprising two or more of the sorbents to remove phospholipids and salts from a sample, to thereby eliminate matrix effects during mass spectrometry analysis. In particular, the sorbents includes at least one sorbent which is water-wettable and contains at least one hydrophobic component and at least one hydrophilic component and at least one of sorbent having a specific affinity for a matrix interference like phospholipids. Further disclosed is a method using the device of the present invention.
Claims95
A separation device comprising two or more sorbents wherein:
- the matrix interferences retained on one of the sorbents is substantially removed by the other sorbents as compared to a separation device comprising only one sorbent;
- the flowrate of the sorbents is substantially improved as compared to a separation device comprising only one sorbent;
- or
- the backpres sure of the sorbents is substantially reduced as compared to a separation device comprising only one sorbent.
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A chromatographic column, comprising:
- a column having a cylindrical interior for accepting a sorbent bed;
- and
- at least two or more sorbents.
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A chromatographic device, comprising:
- a column having a cylindrical interior for accepting a sorbent bed;
- and
- at least two or more sorbents.
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A solid phase extraction method, comprising:
- using a device, wherein the device comprises a sorbent bed including a sorbent containing at least one hydrophobic component and at least one hydrophilic component and a sorbent having a specific affinity to phospholipids.
The method of claim 75, wherein the sorbent that contains at least one hydrophobic component and at least one hydrophilic component is water-wettable.
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Dependencies are inferred from the claim text.