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AssigneesThe United States of America, as represented by the Secretary, Department of Health and Human, The Johns Hopkins University
InventorsJoel Schneider, Gerald Brandacher, Daniel Smith, Gabriel Brat, Johanna Grahammer
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This disclosure provides novel hydrogels that can undergo multiple gel-sol transitions and methods of making and using such hydrogels, particularly in anastomosis procedures. The peptide hydrogels comprising a fibrillar network of peptides that are in an amphiphilic β-hairpin conformation. The peptides comprise photo-caged glutamate residues with a neutral photocage that can be photolytically selectively uncaged to disrupt the fibrillar network and trigger an irreversible gel-sol phase transition of the hydrogel. Isolated peptides for making the disclosed hydrogels are provided, as are methods of using the peptide hydrogels in anastomosis procedures.
A peptide hydrogel comprising a fibrillar network of peptides, wherein:
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An isolated peptide that forms an amphiphilic β-hairpin conformation comprising a β-turn, a first β-strand, a second β-strand, a hydrophobic face, and a hydrophilic face when the peptide is dissolved in an aqueous solution comprising 150 mM NaCl and a pH of 7.4 at 25° C.;
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Dependencies are inferred from the claim text.