Method and process for quantitative evaluation of pharmacokinetics, therapeutic effects and safety of delivery of drug compounds to the inner ear for treatment of auditory disease

A method for treating an auditory disease in a subject in need thereof comprising administering an effective amount of a first pack composition and an effective amount of a second pack composition to the subject, wherein

• (a) the first pack composition comprises about 10 wt. % to about 50 wt. % by weight of the first pack composition of PEG thiol, a PEG thiol-ester, or a mixture thereof and water;
• (b) the second pack composition comprises about 10 wt. % to about 50 wt. % by weight of the second pack composition of a PEG Michael acceptor;
• and water;
• and
• (c) the first pack composition and/or the second pack composition further comprises at least one inner ear-specific therapeutic agent,
• wherein the first pack composition and/or the second pack composition is administered through a cannula that accesses a cochleostomy site or a canalostomy site in the inner ear of the subject;
• and wherein the PEG thiol, PEG thiol-ester, or mixture thereof comprises a multi-arm PEG thiol, a multi-arm PEG thiol-ester, or a mixture thereof.

A hydrogel kit comprising:

• a first pack composition comprising
• about 10 wt. % to about 50 wt. % by weight of the first pack composition of a PEG thiol, a PEG thiol-ester, or a mixture thereof;
• and
• water;
• a second pack composition comprising
• about 10 wt. % to about 50 wt. % by weight of the second pack composition of a PEG Michael acceptor;
• and
• water;
• wherein the PEG thiol, PEG thiol-ester, or mixture thereof comprises a multi-arm PEG thiol, a multi-arm PEG thiol-ester, or a mixture thereof.