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US 2018/0000936 A1Published application
Magnetic extracellular matrix
AssigneeNano3D Biosciences, Inc.
InventorGlauco R. SOUZA
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Abstract
Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.
Claims7
A method of preparing tissue for implantation in a patient, said method comprising:
- a) combining cells with magnetic nanoparticles that are taken up by said cells;
- b) exposing said cells to a magnetic field such that said cells coalesce and levitate;
- c) growing said cells to form a 3D cell culture;
- d) decellularizing said 3D cell culture to make a magnetic ECM;
- e) seeding said magnetic ECM with allogeneic stem or progenitor cells from a patient;
- f) growing said seeded ECM in a magnetic field;
- and
- g) transplanting the seeded ECM from step f) into said patient.
5 more elements
The method of claim 11, wherein said magnetic nanoparticles are provided by blasting, injection, electroporation, magnetic pressure, hydrogels, or cationic liposomes.
A method of making a magnetic ECM, said method comprising:
- a) placing magnetic nanoparticles inside a cell;
- b) levitating said cell in a magnetic field and growing said levitated cell in said magnetic field into a 3D culture of cells having an ECM until said magnetic nanoparticles are lost from said cells and retained by the ECM;
- and
- c) decellularizing said 3D culture of cells using a magnet to levitate and hold said ECM while washing away cell debris to produce a magnetic ECM.
1 more element
Dependencies are inferred from the claim text.