Viral rna segments as immunomodulatory agents and vaccine components
AssigneeUniversity of Iowa Research Foundation
InventorsJack T. STAPLETON, Nirjal BHATTARAI, James MCLINDEN, Jinhau XIANG, Bev DAVIDSON
Life of this patent
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Abstract
The disclosure provides compositions and methods involving viral RNA segments for use in modulating immune responses, including inhibition inflammation related to pathogenic T-cell activation. In addition, modification of the viral sequences responsible for modulating immune response provides for improved vaccine formulations.
Claims30
A method of inhibiting immune cell activation comprising administering to a mammalian subject in need thereof a viral RNA segment comprising a T cell immune-inhibitory domain.
10-12. (canceled)
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A composition comprising a viral RNA segment comprising a T cell immune-inhibitory domain, formulated with a pharmaceutically acceptable carrier buffer or diluent.
24-27. (canceled)
A method of inducing an immune response in a mammalian subject comprising administering to said subject a viral RNA segment wherein said viral RNA segment comprises one or more modified T cell immune-inhibitory domains.
29-44. (canceled)
A vaccine comprising a viral RNA segment having a modification in a T cell immune-inhibitory domain.
46-51. (canceled)
A method of performing gene transfer into a subject comprising administering to said subject
- a) an expression cassette comprising a heterologous gene segment under the control of a promoter operable in cells of said subject and
- b) a viral RNA segment comprising a T cell immune-inhibitory domain.
53-55. (canceled)
A pharmaceutical composition comprising a nucleic acid having the sequence:
- (SEQ ID NO:
- 3)
- CTCACGCCAAGGTGCCTGATCGACTACCCCTACAGGCTCTGGCATTATCC;
- [[or]]
- (SEQ ID NO:
- 4)
- CTCACGCCAAGGTGCCTGATCGACTACCCCTACAGGCTCTGGCATTACCCC
- [[.]];
- or
- (SEQ ID NO:
- 5)
- GACAACAACCTTTACAAACTACATGGT.
- or comprising a nucleic acid sequence selected from GCATTATCC (SEQ ID NO:
- 38), GCAUUAUCC (SEQ ID NO:
- 39), GCATTACCCC (SEQ ID NO:
- 40), GCAUUACCCC (SEQ ID NO:
- 41), CUUUACAAAA (SEQ ID NO:
- 42), or CTTTACAAA (SEQ ID NO:
- 43), wherein said nucleic acid consists essentially of at least about 20 base pairs and no more than about 55 base pairs.
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Dependencies are inferred from the claim text.