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AssigneesThe Broad Institute, Inc., The General Hospital Corporation, Dana-Farber Cancer Institute, Inc.
InventorsNir HACOHEN, Catherine Ju-Ying WU, Edward F. FRITSCH
Estimating the term…
The present disclosure relates to methods for profiling subject specific and personalized T cell receptor (TCR) repertoires using a single-cell sequencing method. More particularly, disclosed are methods for determining binding of T cell receptors to subject specific neoantigens. In addition, the techniques herein may identify the antigenic targets of T cell receptors in the context of tumor neoantigens. Moreover, the present disclosure enables the discovery of T cell targets in numerous diseases, with implications for understanding the basic mechanisms of the mammalian immune response and for developing antigen-specific diagnostic markers and therapies. Finally, cloned TCRs can be used to formulate personalized immunotherapies for those inflicted with a disease, such as cancer.
A method of identifying at least one subject specific T cell receptor pair expressed on a single T cell and preparing a subject-specific immunogenic composition that includes the at least one T cell receptor, wherein the subject has a tumor and the at least one subject-specific T cell receptors are specific to the subject and the subject's tumor, said method comprising:
A method of identifying at least one subject specific T cell receptor pair expressed on a single T cell and preparing a subject-specific immunogenic composition that includes the at least one T cell receptor, wherein the subject has a tumor and the at least one subject-specific T cell receptors are specific to the subject and the subject's tumor, said method comprising:
A method of identifying at least one subject specific T cell receptor pair expressed on a single T cell and preparing a subject-specific immunogenic composition that includes the at least one T cell receptor, wherein the subject has a tumor and the at least one subject-specific T cell receptors are specific to the subject and the subject's tumor, said method comprising:
A method of identifying at least one subject specific T cell receptor pair expressed on a single T cell and preparing a subject-specific immunogenic composition that includes the at least one T cell receptor, wherein the subject has a tumor and the at least one subject-specific T cell receptors are specific to the subject and the subject's tumor, said method comprising:
The method of claim 0, wherein the nucleic acid is a vector.
A method of identifying at least one subject specific TCR expressed on a single T cell, wherein the subject has a tumor and the at least one subject-specific TCRs are specific to the subject and the subject's tumor, said method comprising:
A method of identifying a plurality of at least 4 subject-specific peptides and preparing a subject-specific immunogenic composition that upon administration presents the plurality of at least 4 subject-specific peptides to the subject's immune system, wherein the subject has a tumor and the subject-specific peptides are specific to the subject and the subject's tumor, said method comprising:
A method of treating a subject in need thereof comprising:
A method of treating a subject in need thereof comprising:
A pharmaceutical composition comprising a non-naturally occurring T cell expressing a subject specific TCR pair, wherein the subject specific TCR pair was identified by sequencing of single cells from the subject.
Dependencies are inferred from the claim text.