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US 2018/0000879 A1Published application
Methods of Purifying Cannabinoids, Compositions and Kits Thereof
AssigneePhytoplant Research S.L.
InventorXavier Nadal Roura
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Abstract
The present specification discloses methods of purifying one or more cannabinoids from a plant material, purified cannabinoids and pharmaceutical compositions comprising one or more cannabinoids produced by the disclosed method, methods and uses for treating a disease or condition employing such purified cannabinoids and pharmaceutical compositions.
Claims36
A method of purifying one or more cannabinoids from a plant material, the method consisting essentially of the following steps:
- a) incubating the plant material with a non-polar solvent selected from the group consisting of petroleum ether, pentane and hexane to form a first solvent mixture which extracts the one or more cannabinoids from a plant material;
- b) reducing the volume of the first solvent mixture to about 50% or less of the original volume of the first solvent mixture in step (a) thereby concentrating the one or more cannabinoids;
- c) incubating the reduced first solvent mixture at a temperature range of between about −70° C. to about 40° C. to crystallize the one or more cannabinoids;
- d) incubating the one or more crystallized cannabinoids with the non-polar solvent, wherein the non-polar solvent is the same non-polar solvent as in step (a) to form a second solvent mixture, thereby dissolving at least 50% of the one or more crystallized cannabinoids;
- and
- e) incubating the second solvent mixture at a temperature range of between about −70° C. to about 40° C. to crystallize the one or more cannabinoids, thereby resulting in the purification of one or more cannabinoids.
3 more elements
A method of purifying a cannabinoid from a plant material, the method consisting essentially of the following steps:
- a) incubating the plant material with a solvent selected from the group consisting of pentane, hexane, heptane, cyclohexane, petroleum ethers, dicloromethane, tricloromethane, tethrahydrofurane, diethyl ether, ethanol, methanol, isopropanol, acetone, acetonitrile, ethyl acetate, butane, propane, refrigerant gas 1,1,1,2-Tetrafluoroethane (R134a), liquid CO2, subcritical CO2 or supercritical CO2 or mixes of these solvents to form a first solvent mixture which extracts the one or more cannabinoids from a plant material;
- b) filtering, decanting or centrifuging the first solvent mixture;
- c) reducing the volume of the first solvent mixture to dryness thereby concentrating the one or more cannabinoids in a first extract;
- d) incubating the first extract with a non-polar solvent selected from the group consisting of petroleum ether, pentane and hexane to form a second solvent mixture which extracts the one or more cannabinoids from the first extract;
- e) filtering, decanting or centrifuging the second solvent mixture;
- f) incubating the second solvent mixture at a temperature range of between about −70° C. to about 40° C. to crystallize the one or more cannabinoids;
- g) collecting the one or more crystallized cannabinoids in step (d) using filtration that results in a collection of a crystalline product and a mother liquor;
- h) incubating the one or more crystallized cannabinoids with the non-polar solvent wherein the non-polar solvent is the same non-polar solvent as in step (d) to form a third solvent mixture, wherein the third solvent mixture dissolves at least 50% of the one or more crystallized cannabinoids;
- i) incubating the third solvent mixture at a temperature range of between about −70° C. to about 40° C. to crystallize the one or more cannabinoids;
- and
- j) collecting the one or more crystallized cannabinoids of step (i) using filtration that results in a collection of a crystalline product and a mother liquor, thereby resulting in the purification of one or more cannabinoids
8 more elements
Dependencies are inferred from the claim text.