Stable 5-methyltetrahydrofolate formulations to moderate methylenetetrahydrofolate reductase associated polymorphisms

A method of preparing a solution containing 5-methyltetrahydrofolate retaining a potency of between about 90 to 110% for up to 40 days when stored under refrigeration, comprising the steps of:

• determining the weight of 5-methyltetrahydrofolate powder and sodium metabisulfite;
• determining the water content of the 5-methyltetrahydrofolate;
• adding an excess of between about 5% and 25% of 5-methyltetrahydrofolate powder;
• adding approximately 75% of the total desired volume of sterile water and mixing while keeping the mixture cold;
• mixing an appropriate amount of preservative into the mixture while keeping the mixture cold;
• adding a suitable gentle pH adjuster to adjust the pH to between approximately 3 and 9 while keeping the mixture cold;
• and
• adding enough sterile water to bring the mixture to the desired volume while protecting the mixture from light and keeping the mixture cold, wherein 5-methyltetrahydrofolate or a mixture containing 5-methyltetrahydrofolate is protected from light.