Evaluation of Co-Registered Images of Differently Stained Tissue Slices

A method comprising:

• generating a first lower resolution image from a first higher resolution image of a first tissue slice;
• defining a first shape within the first lower resolution image using digital image analysis;
• generating a second lower resolution image from a second higher resolution image of a second tissue slice;
• defining a second shape within the second lower resolution image using digital image analysis;
• determining that the first shape corresponds to the second shape;
• defining a first region in the first higher resolution image using the first shape within the first lower resolution image;
• defining a second region in the second higher resolution image using the second shape within the second lower resolution image;
• determining co-registration parameters for co-registering the first high resolution digital image with the second high resolution digital image using the first region and the second region;
• and
• displaying a portion of the first high resolution digital image and a portion of the second high resolution digital image in a co-registered orientation using the co-registration parameters.

A method comprising:

• displaying a portion of a digital image of a tissue slice on a graphical user interface, wherein the tissue slice is stained with a biomarker, and wherein the digital image is divided into tile regions;
• generating a statistical value for each of the tile regions of the digital image;
• displaying a tiled representation of the digital image, wherein each of the tile regions of the digital image corresponds to a tile of the tiled representation, wherein each tile has an appearance indicative of the statistical value associated with the tile region corresponding to the tile, and wherein a selected tile corresponds to a first tile region of the digital image that is not visible on the graphical user interface;
• and
• shifting the digital image such that the first tile region becomes visible on the graphical user interface in response to a user selecting the selected tile.

A method comprising:

• displaying a portion of a higher resolution image of a tissue slice on a graphical user interface, wherein the tissue slice is stained with a biomarker, and wherein the higher resolution image is divided into regions;
• generating a statistical value associated with each of the regions;
• generating a lower resolution image from the higher resolution image, wherein each location on the lower resolution image corresponds to a region of the higher resolution image, wherein each location of the lower resolution image has an appearance indicative of the statistical value associated with the corresponding region of the higher resolution image, and wherein a selected location on the lower resolution image corresponds to a first region of the higher resolution image that is not visible on the graphical user interface;
• and
• shifting the higher resolution image on the graphical user interface such that the first region becomes visible on the graphical user interface in response to a user selecting the selected location on the lower resolution image.