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US 2017/0370931 A1Published application
Combination treponemal and non-treponemal syphilis test
AssigneeBio-Rad Laboratories, Inc.
InventorsRavi Kaul, Weiming Zheng, Roger Walker
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Abstract
Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.
Claims20
A kit comprising:
- (i) beads coated with cardiolipin-lecithin-cholesterol antigen;
- and
- (ii) beads coated with Treponema pallidum antigen.
A method of detecting the presence of syphilis antibodies in a sample comprising:
- (a) contacting the sample with (i) beads coated with cardiolipin-lecitin-cholesterol antigen and (ii) beads coated with Treponema pallidum antigen in the same compartment, thereby forming a sample-bead mixture;
- (b) washing the sample-bead mixture to remove unbound sample;
- (c) contacting the sample-bead mixture with a labeled, anti-human immunoglobulin (Ig) antibody;
- and
- (d) detecting the presence of syphilis antibodies by detecting the label on the anti-human Ig antibody.
2 more elements
Dependencies are inferred from the claim text.