Lateral Flow Analyte Detection
AssigneeTGR BioSciences Pty Ltd.
InventorsAntony James Sheehan, Michael Francis Crouch
Life of this patent
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Abstract
Lateral flow methods and apparatuses for detecting one or more analytes are provided. Certain embodiments provide a lateral flow device, kit and method of using the same, comprising: a flow path defined by a permeable sub-assembly of the lateral flow device, a release zone comprising a plurality of peptide-tagged agents, and a detection zone comprising a plurality of anti-peptide agents present in the flow path at a ratio of at least 100:1, on a weight:weight basis, relative to the plurality of peptide-tagged agents.
Claims21
A method to detect a pre-determined analyte, comprising:
- i) exposing a sample comprising the pre-determined analyte to a plurality of peptide-tagged agents to form an analyte complex, said analyte complex comprising the pre-determined analyte and at least one of the plurality of peptide-tagged agents;
- and
- ii) transporting the analyte complex to a detection zone of a lateral flow device, said detection zone comprising a plurality of anti-peptide agents present at a ratio of at least 100:1, on a weight:weight basis, relative to the plurality of peptide-tagged agents, said plurality of anti-peptide agents capable of immobilizing at least one of the at least one of the plurality of peptide-tagged agents;
- and
- iii) detecting the pre-determined analyte.
A method to detect a pre-determined analyte, comprising:
- i) receiving a sample comprising the pre-determined analyte to a permeable release zone of a lateral flow device, said permeable release zone comprising a plurality of peptide-tagged agents and a plurality of detectable agents striped thereon;
- ii) forming an analyte complex comprising the pre-determined analyte bound to at least one of the plurality of peptide-tagged agents and separately bound to at least one of the plurality of detectable agents, wherein the at least one of the plurality of peptide-tagged agents:
- a) is a pre-determined specific binding partner with the pre-determined analyte;
- and
- b) comprises a plurality of covalently conjugated peptide tags;
- iii) transporting the analyte complex by capillary flow action to a detection zone of the lateral flow device, said detection zone comprising a plurality of anti-peptide agents, wherein said plurality of anti-peptide agents are present at a ratio of:
- a) at least 300:1, on a weight:weight basis, relative to the plurality of peptide-tagged agents;
- and
- b) at least 8:1, on a weight:weight basis, relative to the plurality of detectable agents;
- iv) immobilizing the analyte complex to the detection zone by selectively binding at least one of the plurality of anti-peptide agents with at least one of the plurality of covalently conjugated peptide tags;
- and
- v) detecting the immobilized analyte complex within 8 minutes of introducing the sample.
(canceled)
Dependencies are inferred from the claim text.