Expression and secretion system
AssigneeGenentech, Inc.
InventorsDevin TESAR, Xiaocheng CHEN, Mark DENNIS, Isidro HOTZEL
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Abstract
The invention provides an expression and secretion system, and methods of using the same, for the expression and secretion of one fusion protein in prokaryotic cells and a second fusion protein in eukaryotic cells. Also provided herein are nucleic acid molecules, vectors and host cells comprising such vectors and nucleic acid molecules.
Claims16
A nucleic acid molecule encoding a variable heavy chain (VH) domain and a variable light chain (VL) domain and comprising a prokaryotic promoter and/or an eukaryotic promoter which promoters are operably linked to the VH domain and/or VL domain to allow for expression of a VH domain and/or a VL domain in a prokaryotic and a eukaryotic cell, and wherein the VH domain and/or VL is linked to a utility peptide when expressed by a eukaryotic cell and wherein the nucleic acid further encodes a signal sequence which is functional in both a prokaryotic and an eukaryotic cell.
Dependencies are inferred from the claim text.