Reconstruction algorithm for fourier ptychographic imaging

Apparatus for generating an image of a substantially translucent specimen, comprising:

• an imaging system for illuminating and imaging the specimen based on light filtered by an optical element and acquiring a plurality of relatively lower resolution intensity images of the specimen for which content of the images corresponds to partially overlapping regions in frequency space;
• and
• a processor system configured to reconstruct a relatively higher resolution image of the specimen by iteratively updating overlapping regions of the relatively higher resolution image in Fourier space with the plurality of relatively lower resolution intensity images, wherein said iterative updating processes the plurality of relatively lower resolution intensity images in a first sequence which progresses from a centre region of the relatively higher resolution image in increasing spatial frequency followed by a second sequence which progresses towards the centre region in decreasing spatial frequency.

A non-transitory computer readable storage medium having a program recorded thereon, the program being executable by a processor for generating an image of a substantially translucent specimen, the program comprising:

• code for operative for illuminating and imaging the specimen based on light filtered by an optical element to acquire acquiring a plurality of relatively lower resolution intensity images of the specimen for which content of the images corresponds to partially overlapping regions in frequency space;
• and
• code for reconstructing a relatively higher resolution image of the specimen by iteratively updating overlapping regions of the relatively higher resolution image in Fourier space with the plurality of relatively lower resolution intensity images, wherein said iterative updating processes the plurality of relatively lower resolution intensity images in a first sequence which progresses from a centre region of the relatively higher resolution image in increasing spatial frequency followed by a second sequence which progresses towards the centre region in decreasing spatial frequency.