Release reagent for vitamin d compounds
AssigneeRoche Diagnostics Operations, Inc.
InventorsSascha Antoni, Christian Vogl
Life of this patent
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Abstract
A reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein and an in vitro method for the detection of a vitamin D compound in which the vitamin D compound is released from vitamin D-binding protein by the use of this reagent composition as well as the reagent mixture obtained in this manner. Also disclosed is the use of the reagent compositions to release vitamin D compounds as well as a kit for detecting a vitamin D compound.
Claims13
An in vitro method for releasing a vitamin D compound from vitamin D-binding protein comprising the step of:
- a) providing a sample to be investigated and
- b) mixing the sample from step (a) with
- i) a reagent containing one hydrogen carbonate salt and a substance that releases hydrogen carbonate ions (HCO3−) upon hydrolysis, wherein the total concentration of hydrogen carbonate ions (HCO3−) from the hydrogen carbonate salt and released from the substance capable of releasing hydrogen carbonate ions (HCO3−) is 0.1 M to 2.0 M,
- ii) a reducing agent, and
- iii) an alkalinising agent,
- thereby releasing the vitamin D compound from vitamin D-binding protein.
A reagent composition for the release of a vitamin D compound from vitamin D-binding protein comprising one hydrogen carbonate salt and a substance that releases hydrogen carbonate ions (HCO3−) upon hydrolysis, wherein the total concentration of hydrogen carbonate ions (HCO3−) from the hydrogen carbonate salt and released from the substance capable of releasing hydrogen carbonate ions (HCO3−) is 0.1 M to 2.0 M, and a reducing agent.
Dependencies are inferred from the claim text.