Biosensors Engineered from Conditionally Stable Ligand-Binding Domains
OwnerGeorge M. Church, Justin Feng +5inventors
InventorsGeorge M. Church, Justin Feng, Daniel J. Mandell, David Baker, Stanley Fields, Benjamin Ward Jester, Christine Elaine Tinberg
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Abstract
Disclosed is a biosensor engineered to conditionally respond to the presence of specific small molecules, the biosensors including conditionally stable ligand-binding domains (LBDs) which respond to the presence of specific small molecules, wherein readout of binding is provided by reporter genes or transcription factors (TFs) fused to the LBDs.
Claims19
A biosensor comprising a ligand binding domain (LBD) engineered to respond conditionally to the presence of specific small molecules, the biosensor including an LBD that is fused to a reporter protein or transcription factor (TF).
A biosensor comprising an engineered digoxigenin-binding protein, DIG10.3, fused to the DNA-binding domain of Gal4 and a VP16 activation domain.
Dependencies are inferred from the claim text.