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AssigneeMemorial Sloan Kettering Cancer Center
InventorsLorenz Studer, Daniela Cornacchia
Estimating the term…
Provided are age-modified cells and method for making age modified cells by reducing or increasing the level of genomic nucleic acid methylation in the cells. The aging and/or maturation process can be accelerated or reduced and controlled for young, aged, mature and/or immature cells, such as a somatic cell, a stem cell, a stem cell-derived somatic cell, including an induced pluripotent stem cell-derived cell, by reducing or increasing the level of genomic nucleic acid methylation in the cells. Methods described by the present disclosure can produce age-appropriate cells from a somatic cell or a stem cell, such as an old cell, young cell, immature cell, and/or a mature cell. Such age-modified cells constitute model systems for the study of late-onset diseases and/or disorders.
A method for producing a cell exhibiting one or more chronological marker, said method comprising:
A cell exhibiting at least one chronological marker induced by reducing the level of genomic nucleic acid methylation in a cell in an amount and for a period of time sufficient to induce said at least one chronological marker.
A method for drug screening, said method comprising
A method for reducing the expression level of at least one chronological marker in a cell, said method comprising:
A cell prepared by increasing the level of genomic nucleic acid methylation in the cell, wherein the level of methylation is increased in an amount and for a period of time sufficient to reduce expression of at least one chronological marker.
A method for determining the molecular age of a cell comprising determining the ratio of expression levels of one or more Line1 (L1), LTR, and/or ERV repetitive elements to one or more ALU repetitive elements in the cell, wherein a ratio greater than 1 is indicative of the cell having an aged or old molecular status.
A kit for producing a cell exhibiting one or more chronological marker, said kit comprising an agent that reduces genomic nucleic acid methylation in a cell.
Dependencies are inferred from the claim text.