Patterned neuromuscular junctions and methods of use
AssigneeLos Alamos National Security, LLC
InventorsRashi Iyer, Piyush Bajaj
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Abstract
Disclosed herein are devices including a substrate, one or more regions of electrically active material, and optionally at least one electrode. Also disclosed herein are devices or systems including a substrate, one or more regions of electrically active material including muscle cells and neuronal cells, and optionally at least one electrode. In some embodiments, the muscle cells and neuronal cells form one or more neuromuscular junctions at defined locations on the electrically active material. Also disclosed are methods of using the devices and systems for analyzing the effect of compounds on neuronal cell, muscle cell and/or neuromuscular junction activity.
Claims34
A system, comprising:
- a substrate comprising one or more regions of a biocompatible electrically active material disposed on the substrate wherein the regions of the electrically active material comprise at least one first area and at least one second area, wherein the second area is perpendicular or radial to the first area and is in physical contact with the first area;
- at least one electrode connected to each of the one or more regions of electrically active material;
- a plurality of muscle cells and a plurality of neuronal cells, wherein the muscle cells and neuronal cells are present on the one or more regions of the electrically active material;
- and
- one or more neuromuscular junctions between the neuronal cells and muscle cells.
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A device, comprising:
- a substrate comprising one or more regions of a biocompatible electrically active material disposed on the substrate wherein the regions of the electrically active material comprise at least one first area and at least one second area, wherein the second area is perpendicular or radial to the first area and is in physical contact with the first area;
- at least one electrode connected to each of the one or more regions of electrically active material;
- and
- a plurality of muscle cells and a plurality of neuronal cells on the one or more regions of the electrically active material.
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Dependencies are inferred from the claim text.