Charge reversible ion exchange resins, chromatography column, method, and system thereof
AssigneeDionex Corporation
InventorChristopher A. POHL
Life of this patent
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Abstract
An ion exchange chromatographic packing material is described that includes a copolymer grafted to support resin particles. The copolymer includes an ion exchange group, an ionic crosslinking group configured to ionically bind to the ion exchange group, and an adjustable ionization state group having at least a first net charge at the first pH and a second net charge at the second pH. An overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to the overall second net charge of the chromatographic packing material. This allows impurities to be removed from the chromatographic packing material at the second pH.
Claims42
An ion exchange chromatographic packing material comprising:
- a) support resin particles;
- and
- b) a copolymer grafted to the support resin particles, the copolymer comprising:
- i) an ion exchange group configured to have a polarity that does not change when switching from a first pH to a second pH, in which the first pH and the second pH are different,
- ii) an ionic crosslinking group configured to ionically bind to the ion exchange group,
- iii) an adjustable ionization state group having at least a first net charge at the first pH and a second net charge at the second pH, in which the first net charge and the second net charge are different,
- where an overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to the overall second net charge of the chromatographic packing material.
An ion exchange chromatographic packing material made by a method comprising:
- combining a support resin particle, an ion exchange monomer, an ionic crosslinking monomer, and an adjustable ionization state monomer to form a reaction mixture;
- forming a copolymer of the ion exchange monomer, the ionic crosslinking monomer, and the adjustable ionization state monomer;
- and
- grafting the copolymer to the support resin particle,
- in which a polarity of the ion exchange monomer does not change when switching from a first pH to a second pH, in which the first pH and the second pH are different, in which the ionic crosslinking monomer is configured to ionically bind to the ion exchange monomer, in which the adjustable ionization state monomer has at least a first net charge at the first pH and a second net charge at the second pH, where an overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to the overall second net charge of the chromatographic packing material.
A method of separating an analyte, the method comprising:
- flowing a first eluent having a first pH and containing a plug of a sample into a chromatography column containing a packed bed of the ion exchange chromatographic packing material, the ion exchange chromatographic packing material comprising:
- a) support resin particles;
- and
- b) a copolymer grafted to the support resin particles, the copolymer comprising:
- i) an ion exchange group configured to have a polarity that does not change when switching from a first pH to a second pH, in which the first pH and the second pH are different,
- ii) an ionic crosslinking group configured to ionically bind to the ion exchange group,
- iii) an adjustable ionization state group having at least a first net charge at the first pH and a second net charge at the second pH, in which the first net charge and the second net charge are different,
- where an overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to the overall second net charge of the chromatographic packing material;
- separating the sample into one or more analyte bands in the chromatography column;
- and
- detecting the one or more analyte bands eluting off of the chromatography column.
A system for chromatographically separating a sample, the system comprising:
- a pump;
- an injection valve;
- a chromatography column containing a packed bed of the ion exchange chromatographic packing material, the ion exchange chromatographic packing material comprising:
- a) support resin particles;
- and
- b) a copolymer grafted to the support resin particles, the copolymer comprising:
- i) an ion exchange group configured to have a polarity that does not change when switching from a first pH to a second pH, in which the first pH and the second pH are different,
- ii) an ionic crosslinking group configured to ionically bind to the ion exchange group,
- iii) an adjustable ionization state group having at least a first net charge at the first pH and a second net charge at the second pH, in which the first net charge and the second net charge are different,
- where an overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to the overall second net charge of the chromatographic packing material;
- and
- a detector.
Dependencies are inferred from the claim text.