System for capillary electrophoresis for peptide and protein analysis
AssigneeUniversity of Notre Dame du Lac
InventorsNorman DOVICHI, Guijie ZHU, Liangliang SUN
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Abstract
The invention provides a novel method of coating the inside of a capillary with a polymeric material. The method can include introducing a catalyst-free solution of a monomer and initiator, wherein the monomer is present in about 1-10% (w/v) and the initiator is present in 0.1-1% (w/v), into a capillary and thermally initiating polymerization of the monomer thereby providing a capillary comprising an internal polymeric coating for separating, identifying, and quantifying components of an analyte.
Claims20
A method of coating the inside of a capillary with a polymeric material comprising introducing a catalyst-free solution of a monomer and initiator, wherein the monomer is present at about 2% (w/v) to about 10% (w/v) and the initiator is present at about 0.1% (w/v) to about 1% (w/v), into a capillary and thermally initiating polymerization of the monomer thereby providing a capillary comprising an internal polymeric coating.
The method of 1 wherein the initiator comprises a persulfate.
A method for separating, identifying, and quantifying components of an analyte comprising introducing the analyte to a capillary, wherein the capillary has a proximate end and a distal end and an interior wherein the interior of the capillary has been coated with a polymeric coating, the polymeric coating introduced via vacuum into the capillary as a solution of a monomer and an initiator and polymerization of the monomer initiated thermally inside the capillary, migrating the analyte through the capillary, and detecting analyte components.
Dependencies are inferred from the claim text.