Devices and methods for measuring the properties of macromolecules

A method of fabricating a macromolecule measurement device, comprising:

• providing a macromolecule measurement unit, wherein the macromolecule measurement unit comprises an electronic sensing structure, an interface structure overlying the electronic sensing structure, and a macromolecule measurement structure overlying the interface structure;
• providing a fluid control unit wherein the fluid control unit comprises a carrier, a release layer overlying the carrier, and a fluid control structure overlying the release layer;
• and
• bonding the fluid control structure to the macromolecule measurement structure.

A device for the measurement of a macromolecule, comprising:

• a measurement chamber, wherein the measurement chamber comprises an electrode comprising a molecule capable of interacting with a macromolecule to cause a change in an response detectable by the electrode;
• a reaction chamber fluidly coupled to the measurement chamber and fluidly coupled to one or more microfluidic channels;
• and
• a magnet configured to produce a magnetic field in the reaction chamber.

A method for measuring a macromolecule, comprising:

• introducing magnetic particles into a reaction chamber, wherein the magnetic particles comprise a molecule configured to bind cells of a biological sample to the magnetic particles;
• immobilizing the magnetic particles in the reaction chamber with a magnetic field;
• exposing the immobilized magnetic particles to the biological sample to bind the cells;
• washing the immobilized magnetic particles comprising the bound cells;
• exposing the bound cells to a chemical to produce a solution comprising a macromolecule expressed by the cell;
• introducing the solution comprising the expressed macromolecule into a measurement chamber;
• and
• measuring the macromolecule in the measurement chamber.