Electrodes Having at Least One Sensing Structure and Methods for Making and Using the Same
AssigneeAbbott Diabetes Care Inc.
InventorsUdo Hoss, Tahir Sadik Khan, Phu X. Le
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Abstract
Embodiments of the present disclosure relate to electrochemical analyte sensor electrodes that have one or more sensing structures, each structure has a respective perimeter at least partially around it to define the structure so that each of the structures have a liquid limiting barrier around their perimeters. The liquid limiting perimeter may completely or partially encompass the perimeter of each sensing structure of the electrode. Also provided are methods for fabricating the electrodes, analyte sensors employing the subject electrodes, and methods of using the analyte sensors in analyte monitoring.
Claims90
An electrode comprising:
- a non-conductive material;
- a conductive layer disposed on the material;
- and
- a sensing structure on the conductive layer comprising a removed portion of conductive material forming a fluid barrier perimeter;
- and
- reagent comprising analyte responsive enzyme positioned within the sensing structure.
The electrode according to any one of the previous claims, wherein the fluid barrier perimeter has a depth from 1 μm to 25 μm into the material.
The electrode according to any one of the previous claims, wherein the conductive layer comprises a material selected from the group consisting of silver, indium tin oxide, gold, platinum, copper, nickel, rhodium, ruthenium, ruthenium dioxide, cobalt, zinc, titanium, palladium, carbon, platinum-carbon and conductive polymer.
The electrode according to any one of the previous claims, wherein the conductive layer has a thickness of from 50 μm to 200 μm.
The electrode according to any one of the previous claims, wherein the sensing structures have an average diameter of 200 μm or less.
The electrode according to any one of the previous claims, further comprising a membrane disposed over conductive layer.
The electrode according to any one of the previous claims, wherein the analyte-responsive enzyme comprises a glucose-responsive enzyme.
The electrode according to any one of the previous claims, wherein the reagent further comprises a redox mediator.
A method for monitoring a level of an analyte in a subject, the method comprising:
- positioning at least a portion of an analyte sensor into a skin of a subject, wherein the analyte sensor comprises:
- a working electrode;
- and
- a counter electrode,
- wherein the working electrode comprises:
- a non-conductive material;
- a conductive layer disposed on the material;
- and
- a sensing structures on the conductive layer comprising a removed portion of conductive material forming a fluid barrier perimeter;
- and
- reagent comprising analyte responsive enzyme positioned within the sensing structure;
- and
- determining a level of an analyte over a period of time from signals generated by the analyte sensor,
- wherein the determining over a period of time provides for monitoring the level of the analyte in the subject.
A method of fabricating an electrode, the method comprising:
- applying a conductive material to a surface of a material to form a conductive layer;
- removing a portion of the conductive layer to define an electrode on the surface of the material;
- removing a portion of the conductive layer within the electrode to form a sensing structure having a removed portion of conductive material forming a fluid barrier perimeter around the sensing structure;
- and
- depositing a reagent comprising an analyte responsive enzyme within the sensing structure of the electrode.
Dependencies are inferred from the claim text.