Treatment of angiogenesis disorders
AssigneeThe Rockefeller University
InventorsSohail Tavazoie, Nils Halberg, Kim Png
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Abstract
This invention concerns pathological angiogenesis and cancer, related treatment methods, and related compositions. Also disclosed are related diagnosis kits and methods.
Claims8
A method for generating a population of mammalian cancer cells with increased metastatic tissue colonization potential, comprising performing serial rounds of transplantation, isolation, and repeat transplantation of a population of labeled or unlabeled cancer cells into a living tissue.
A method for identifying a gene or a non-coding RNA that regulates metastatic cancer colonization of a body tissue, comprising,
- a) introducing one or more shRNA, RNAi, microRNA, or non-coding RNA molecules into a population of starting cancer cells to generate a population of engineered cancer cells;
- b) transplanting said population of engineered cancer cells into a tissue of the body;
- c) isolating transplanted cells to obtain a population of isolated cancer cells;
- and
- d) assessing the quantity of the shRNA, RNAi, microRNA, or non-coding RNA molecule or protein encoding gene in the population of isolated cancer cells,
- wherein a decrease in the quantity of said shRNA, RNAi, microRNA, or non-coding RNA in the population of isolated cells relative to its quantity prior to transplantation indicates that the target gene or genes of said shRNA, RNAi, microRNA, or non-coding RNAs represents a gene required for metastatic colonization of said tissue.
A method for identifying a gene or a non-coding RNA that regulates metastatic cancer colonization of a body tissue, comprising
- a) introducing one or more DNA molecules that encode a protein encoding gene into a population of starting cancer cells to generate a population of engineered cancer cells;
- b) transplanting said population of engineered cancer cells into a tissue of the body;
- c) isolating transplanted cells to obtain a population of isolated cancer cells;
- and
- d) assessing the quantity of the protein encoding gene in the population of isolated cancer cells,
- wherein an increase in the quantity of said protein encoding gene in the population of isolated cells relative to its quantity prior to transplantation indicates that the protein encoding gene is required for metastatic colonization of said tissue.
A composition comprising a population of metastatic human colon cancer cells with an increased potential to colonize the liver.
Dependencies are inferred from the claim text.