Novel compositions, methods and kits for microorganism detection
AssigneeLife Technologies Corporation
InventorsIoanna PAGANI, Pius BRZOSKA, Kelly LI, Sunali PATEL, Boli HUANG, Kamini VARMA, Nitin PURI, Evan DIAMOND
Life of this patent
Estimating the term…
Abstract
Methods, compositions and kits for detecting microorganisms and/or profiling microbiota such as for example through use of nucleic acid amplification and detection.
Claims35
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A method for detecting the presence of a microorganism nucleic acid in a sample, said method comprising:
- (a) distributing portions of a nucleic acid sample to individual reaction chambers situated within a support;
- (b) performing parallel amplification reactions and forming amplification products in individual reaction chambers, wherein each amplification reaction contains a pair of amplification primers configured to produce an amplification product corresponding to said target nucleic acid sequence present within, or derived from, the genome of a microorganism, wherein said amplification product comprises a sequence listed in Table 4 (i.e., SEQ ID NOs:1 through 34);
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- (c) determining whether said amplification product has been formed in one or more of said individual reaction chambers.
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A composition for determining the presence or absence of at least one target nucleic acid from one or more of the microorganisms listed in Table 1 in a biological sample, said composition comprising:
- (a) at least one amplification primer pair, wherein each of said primers of said pair comprises a target hybridization region that is configured to specifically hybridize to all or a portion of a region of said target nucleic acid and wherein under suitable conditions said primer pair generates an amplicon which comprises a sequence listed in Table 4 (i.e., SEQ ID NOs:1 through 34);
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- (b) at least one detection probe configured to specifically hybridize to all or a portion of a region of said amplicon produced by said primer pair.
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A method of profiling gene expression in a human subject, said method comprising determining, for a set of genes, a level of RNA or DNA encoded by said genes in a biological sample of said subject, wherein said set comprises the genes identified as those listed in Table 2 or any combination thereof.
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Dependencies are inferred from the claim text.