Method and device for determining the presence of a micro-organism in stools with activated carbon pretreatment

A device (I, 1) for detecting at least one antigen of a target microorganism in a liquid sample (3), the device comprising:

• a) a support (100);
• and
• b) a porous diffusion medium (2, 200) fixed on the support (100), and enabling the liquid sample (3) to migrate, said diffusion medium (2, 200) comprising:
• i) an application zone (10, 300) for applying the liquid sample (3);
• ii) a purification zone (20, 300) including activated carbon (21, 301);
• iii) a marking zone (30, 500) including at least a first marked binding partner, said first binding partner being capable of binding with said at least one antigen of the microorganism that is to be detected, if present in the liquid sample, then forming a first binding partner and antigen complex;
• and
• iv) at least one reaction zone (40, 800) comprising:
• a display zone (41, 600) for displaying the results of the detection and comprising at least one second binding partner held stationary on the diffusion medium and suitable for binding with said first binding partner and antigen complex;
• a migration verification zone (42, 700) enabling proper operation of the device to be verified, which zone is situated downstream from the display zone (41, 600);
• and
• said application zone (10, 300), purification zone (20, 300), marking zone (30, 500), and reaction zone (40, 800) being in communication to enable the liquid to diffuse.