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US 2017/0362600 A1Published application
Methodologies and Compositions for Creating Targeted Recombination and Breaking Linkage Between Traits
AssigneeDow AgroSciences LLC
InventorsSandeep Kumar, Andrew F. Worden, Stephen Novak, Ryan M. Lee
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Abstract
The current disclosure relates to methods and compositions for improving plant varieties through plant breeding and plant genetics. For instance, the disclosure concerns increasing the recombination frequency of a heterozygous trait genetically linked to a second trait within plants. Further, the disclosure concerns breaking the genetic linkage between a first allele and a second allele.
Claims25
A method for increasing the frequency of genetic recombination between a first locus genetically linked to a second locus within a genome of a plant, the method comprising the steps of:
- a) introducing a site specific nuclease into the genome of the plant;
- b) producing a double stranded break with the site specific nuclease in one of two homologous chromosomes;
- c) undergoing recombination within the plant genome;
- and,
- d) modifying the plant genome, wherein the modified plant genome comprises increased frequency of genetic recombination between the first locus and the second locus.
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