Host Cell Modification with Artificial Endosymbionts
AssigneeBell Biosystems, Inc.
InventorsCaleb B. Bell, III, Alexey Bazarov, Abdul Wakeel, Joyce Barrozo
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Abstract
The present invention is directed generally to host cells with artificial endosymbionts, wherein the artificial endosymbiont and the host cell communicate with each other to alter a phenotype of the host cell. In some embodiments, the communication comprises the secretion of a polypeptide from the artificial endosymbiont into the host cell. The secreted polypeptide can be a selectable marker, a reporter protein, a transcription factor, a signal pathway protein, a receptor, a growth factor, a cytokine, an effector molecule or other factors that can produce a phenotype in the host cell.
Claims36
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An isolated mammalian cell comprising a magnetotactic bacterium, wherein the magnetotactic bacterium provides a protein to the host cell and thereby provides a phenotype to the mammalian cell, and wherein the mammalian cell is a hematopoietic cell.
A method for introducing a phenotype into a mammalian cell, comprising the steps of:
- culturing an isolated mammalian cell comprising a magnetotactic bacterium wherein the mammalian cell is a hematopoietic cell;
- expressing a protein in the magnetotactic bacterium;
- secreting the protein from the magnetotactic bacterium to the hematopoietic cell whereby the protein produces a phenotype in the hematopoietic cell.
Dependencies are inferred from the claim text.