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US 2017/0362567 A1Published application
Methods for storage and retrieval of functional mature retinal cells
AssigneeMorehouse School of Medicine
InventorsPeter R. MACLEISH, Xiaoming Chen
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Abstract
A method for the isolation, storage and retrieval of mature retinal cells is disclosed. The Method is applicable to adult mammalian cone cells, and more particularly human cone cells, and to healthy as well as pathological or otherwise altered cone cells. A kit for the isolation, storage and retrieval of mature retinal cells is also described.
Claims22
1-16. (canceled)
A method for treating retinal dysfunction in a subject, comprising:
- introducing into an eye of said subject an effective amount of retinal cone cells stored and retrieved using a method comprising the steps of:
- incubating freshly isolated retinal tissue with a retinal tissue digestion solution comprising a protease and CaCl2;
- removing said retinal tissue digestion solution to yield an enzyme-treated retinal tissue;
- triturating said enzyme-treated retinal tissue in a retinal cell suspension medium to form a retinal cell suspension comprising retinal cone cells;
- adding fetal bovine serum (FBS) and dimethyl sulfoxide (DMSO) to said retinal cell suspension;
- freezing and storing said retinal cell suspension comprising FBS and DMSO in liquid nitrogen;
- thawing said retinal cell suspension;
- washing and culturing said thawed retinal cells with a retinal cell culture medium comprising Choline Cl;
- and
- isolating mature retinal cone cells recovered from said frozen retinal cell suspension.
7 more elements
(canceled)
Dependencies are inferred from the claim text.