Tem-1 diagnostic antibodies
AssigneeMorphotek, Inc.
InventorDaniel John O'Shannessy
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Abstract
Described herein are anybodies, and antigen-binding fragments thereof, that are specific for tumor endothelial marker 1 (TEM-1), related polynucleotides, expression vectors, and cells that express the described antibodies. Also provided are methods of using the described antibodies, and antigen-binding fragments thereof, and related kits. Provided herein are also methods for diagnosing TEM-1-expressing cancers (such as melanoma, sarcoma, bladder cancer or colon cancer) using the described antibodies, and antigen-binding fragments thereof. The methods involve determining the amount of TEM-1 in a biological sample derived from a subject and comparing this level with the level of TEM-1 in a known standard or reference sample.
Claims19
A chimeric antibody, or antigen-binding fragment thereof, that specifically binds TEM-1 comprising a light chain CDR1 having the amino acid sequence of SEQ ID NO:
- 73, a light chain CDR2 having the amino acid sequence of SEQ ID NO:
- 74, a light chain CDR3 having the amino acid sequence of SEQ ID NO:
- 75, a heavy chain CDR1 having the amino acid sequence of SEQ ID NO:
- 77, a heavy chain CDR2 having the amino acid sequence of SEQ ID NO:
- 78, and a heavy chain CDR3 having the amino acid sequence of SEQ ID NO:
- 79.
An isolated polynucleotide sequence encoding an antibody light chain, or antigen-binding fragment thereof, of an antibody that that specifically binds to TEM-1, wherein the light chain CDR1 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 57, the light chain CDR2 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 58, and the light chain CDR3 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 59.
An isolated polynucleotide sequence encoding an antibody heavy chain, or antigen-binding fragment, thereof, of an antibody that that specifically binds to TEM-1, wherein the heavy chain CDR1 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 61, the heavy chain CDR2 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 62, and the heavy chain CDR3 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 63.
An isolated polynucleotide sequence encoding an antibody light chain, or antigen-binding fragment thereof, of an antibody that that specifically binds to TEM-1, wherein the light chain CDR1 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 73, the light chain CDR2 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 74, and the light chain CDR3 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 75.
An isolated polynucleotide sequence encoding an antibody heavy chain, or antigen-binding fragment thereof, of an antibody that that specifically binds to TEM-1, wherein the heavy chain CDR1 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 77, the heavy chain CDR2 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 78, and the heavy chain CDR3 of the encoded antibody comprises the amino acid sequence of SEQ ID NO:
- 79.
A method of detecting TEM-1 in a biological sample, comprising exposing the sample to the antibody, or antigen-binding fragment thereof, that specifically binds TEM-1 comprising a light chain CDR1 having the amino acid sequence of SEQ ID NO:
- 57, a light chain CDR2 having the amino acid sequence of SEQ ID NO:
- 58, a light chain CDR3 having the amino acid sequence of SEQ ID NO:
- 59, a heavy chain CDR1 having the amino acid sequence of SEQ ID NO:
- 61, a heavy chain CDR2 having the amino acid sequence of SEQ ID NO:
- 62, and a heavy chain CDR3 having the amino acid sequence of SEQ ID NO:
- 63, and detecting TEM-1.
A method of detecting TEM-1 in a biological sample, comprising exposing the sample to the antibody, or antigen-binding fragment thereof, that specifically binds TEM-1 comprising a light chain CDR1 having the amino acid sequence of SEQ ID NO:
- 73, a light chain CDR2 having the amino acid sequence of SEQ ID NO:
- 74, a light chain CDR3 having the amino acid sequence of SEQ ID NO:
- 75, a heavy chain CDR1 having the amino acid sequence of SEQ ID NO:
- 77, a heavy chain CDR2 having the ammo acid sequence of SEQ ID NO:
- 78, and a heavy chain CDR3 having the amino acid sequence of SEQ ID NO:
- 79, and detecting TEM-1.
Dependencies are inferred from the claim text.