Humanized anti-alpha v beta 5 antibodies and uses thereof
OwnerThomas Owen Cameron, Michael Adam Crackower +8inventors
InventorsThomas Owen Cameron, Michael Adam Crackower, Brian M. Dolinski, Karl J.M. Hanf, Amy Theresa McCurley, Nels Eric Pederson, Martin Preyer, Fang Qian, Shelia M. Violette, Paul Henry Weinreb
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Abstract
Humanized antibodies and antibody fragments that bind to αvβ5 are disclosed. Also disclosed are methods of using the disclosed antibodies and antibody fragments to treat or prevent αvβ5-mediated diseases.
Claims32
1-22. (canceled)
An antibody or antigen-binding fragment thereof that specifically binds to αvβ5, wherein the antibody or the antigen-binding fragment thereof comprises:
- a heavy chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:1 and a light chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:8, 9, 10, 11, or 12;
- a heavy chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:2 and a light chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:8, 9, 10, 11, or 12;
- a heavy chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:3 and a light chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:8, 9, 10, 11, or 12;
- a heavy chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:4 and a light chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:8, 9, 10, 11, or 12;
- a heavy chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:5 and a light chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:8, 9, 10, 11, or 12;
- a heavy chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:6 and a light chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:8, 9, 10, 11, or 12;
- or
- a heavy chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:7 and a light chain variable region that comprises the amino acid sequence set forth in SEQ ID NO:8, 9, 10, 11, or 12.
An antibody comprising the amino acid sequence set forth in
- (i) SEQ ID NO:69 and 70;
- (ii) SEQ ID NO:69 and 82;
- (iii) SEQ ID NO:80 and 82;
- or
- (iv) SEQ ID NO:81 and 70.
An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs:95 to 102, 34, and 53 to 55.
(canceled)
An isolated nucleic acid comprising a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:1-7, 8-12, 69, 70, 80, 81, and 82.
(canceled)
A method of preparing a humanized antibody comprising culturing a host cell comprising recombinant vectors comprising:
- the nucleic acid sequences set forth in SEQ ID NOs:99 and 53;
- the nucleic acid sequences set forth in SEQ ID NOs:99 and 102;
- the nucleic acid sequences set forth in SEQ ID NOs:97 and 102;
- the nucleic acid sequences set forth in SEQ ID NOs:100 and 53;
- or
- the nucleic acid sequences set forth in SEQ ID NOs:99 and 34;
- under conditions appropriate for expression of a humanized antibody, wherein humanized antibody chains are expressed and a humanized antibody is produced.
Dependencies are inferred from the claim text.