Oligopeptide-free cell culture media
AssigneeBaxalta Incorporated
InventorsLeopold Grillberger, Manfred Reiter, Wolfgang Mundt, Artur Mitterer
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Abstract
The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.
Claims21
An oligopeptide-free cell culture medium, comprising at least 0.5 mg/L of a polyamine.
A method for expressing at least one protein, comprising the steps of:
- (a) providing a culture of cells;
- (b) introducing at least one nucleic acid sequence comprising a sequence coding for at least one protein into the cells;
- selecting the cells carrying the nucleic acid sequence;
- and
- expressing the protein in the cells in a medium comprising at least 0.5 mg/L of a polyamine.
A method for producing at least one virus, comprising the steps of:
- (a) providing a culture of cells;
- (b) infecting the cells with the at least one virus;
- (c) selecting the virus-infected cells;
- and
- (d) propagating the at least one virus in the cells in a medium comprising at least 0.5 mg/L of a polyamine.
Dependencies are inferred from the claim text.