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US 2017/0362272 A1Published application
Protein manufacture
AssigneeUcb Biopharma Sprl
InventorsPhilip BASSETT, Richard DAVIES, Elena GONZALEZ, Mark PEARCE-HIGGINS
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Abstract
The present invention provides a novel method for protein manufacture wherein the protein is expressed in a host cell, and in a more specific manner relates to a method for manufacturing a protein that results in reduced levels of product-related impurities.
Claims16
1-14. (canceled)
A method for manufacturing a protein of interest wherein said protein is expressed in a host cell, comprising:
- a) culturing said host cells under conditions such that they express said protein,
- b) collecting the host cells from the cell culture fluid,
- c) adding buffer to said host cells, and
- d) subjecting the host cells to heat treatment wherein the redox potential of said buffer is maintained below −0 mV during said heat treatment.
1 more element
Dependencies are inferred from the claim text.