Cartilage compositions and methods for modifying proteoglycan content
AssigneeISTO Technologies, Inc.
InventorsNeil Kizer, Nicole M. Bergmann, Gary Gage, Huston Davis Adkisson, Michael Maloney
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Abstract
The present disclosure relates to cartilage repair compositions and methods for modifying the proteoglycan content of the compositions. Specifically, the methods relate to serum free, collagen free neocartilage made from chondrocytes that can be used for implants. Proteoglycans, such as aggrecan and sulfated glycosaminoglycan are used and the content modified using temperature changes.
Claims45
A cartilage replacement composition comprising:
- neocartilage maintained at a temperature below physiologic temperature for a period of time sufficient for the proteoglycan content of the neocartilage to decrease relative to the proteoglycan content of the neocartilage at a physiological temperature, wherein the neocartilage at the temperature of about 30° C. or lower is capable of increasing the proteoglycan content when returned to a physiological temperature.
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A method for modifying the proteoglycan content of a cartilage composition for the repair of cartilage tissue, said method comprising:
- a) obtaining the cartilage composition, wherein the cartilage composition has previously been maintained at a physiological temperature and has an initial proteoglycan content;
- b) sealing the cartilage composition in a container in a serum-free medium;
- c) maintaining the cartilage composition in the sealed container at a storage temperature below physiologic temperature for a storage period of time sufficient for the proteoglycan content of the cartilage composition to decrease to a storage proteoglycan content less than the initial proteoglycan content;
- d) removing the cartilage composition from the container and the storage temperature and returning the cartilage composition to recovery conditions comprising a serum-free medium and a physiological temperature;
- e) maintaining the cartilage composition under the recovery conditions for a period of time sufficient for the proteoglycan content of the cartilage composition to increase to a recovery proteoglycan content.
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A method for of repairing cartilage tissue of a subject, the method comprising:
- a) obtaining a cartilage composition prepared by a process comprising sealing the cartilage composition in a container in a serum-free medium, and maintaining the cartilage composition in the sealed container at a storage temperature below physiologic temperature for a storage period of time sufficient for the proteoglycan content of the cartilage composition to decrease to a storage proteoglycan content less than the initial proteoglycan content;
- b) removing the cartilage composition from the container and the storage temperature;
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- c) implanting the cartilage composition in a cartilage defect of the subject, wherein the implantation returns the cartilage composition to a physiological temperature;
- d) maintaining the cartilage composition in the defect for a period of time sufficient for the proteoglycan content of the cartilage composition to increase to a recovery proteoglycan content of the composition.
Dependencies are inferred from the claim text.