Polypeptide complex as polypeptide or protein drug carrier, method of using the polypeptide complex, and fusion protein complex thereof
AssigneeTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
InventorsPeijian ZOU, Huanbo TAN, Wencheng SU, Jibin SUN
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Abstract
The present invention provides a polypeptide complex on the basis of Titin-Telethonin beta-pleated sheet structure as a polypeptide or protein drug carrier, a method of using the polypeptide complex, and a fusion protein complex thereof. The polypeptide complex is capable of maintaining the activity of polypeptide or protein drugs and prolonging the half-life period simultaneously.
Claims25
A polypeptide complex as a polypeptide or protein drug carrier, comprising:
- (i) a polypeptide A, which is a polypeptide comprising two Ig domains at an N terminus of a titin molecule, or a homologue, an analogue or a derivative thereof;
- and
- (ii) a polypeptide B, which is a polypeptide comprising a beta-pleated sheet region at an N terminus of a Telethonin molecule, or a homologue, an analogue or a derivative thereof,
- wherein the polypeptide B binds two polypeptides A together to form a beta-pleated sheet structure.
A fusion protein complex with a therapeutic effect, comprising a fusion protein formed by a polypeptide or protein drug with a polypeptide A and/or a polypeptide B, and the fusion protein forms a beta-pleated sheet structure with the polypeptide A, the polypeptide B, or an additional fusion protein,
- wherein the polypeptide A is a polypeptide comprising two Ig domains (Z1Z2) at an N terminus of a titin molecule, or a homologue, an analogue or a derivative thereof;
- and the polypeptide B is a polypeptide comprising a beta-pleated sheet region at an N terminus of a Telethonin molecule, or a homologue, an analogue or a derivative thereof.
A fusion protein with a therapeutic effect, comprising a first polypeptide A, a polypeptide B, and a second polypeptide A linked directly or indirectly in sequence, and also one or more polypeptide or protein drugs inserted in or attached to one or more sites in any of the first polypeptide A and the second polypeptide A and/or the polypeptide B, the first polypeptide A and the second polypeptide A and the polypeptide B forms a beta-pleated sheet structure by a flexible linker,
- wherein the first polypeptide A and the second polypeptide A are polypeptides comprising two Ig domains (Z1Z2) at an N terminus of a titin molecule, or a homologue, an analogue or a derivative thereof;
- and the polypeptide B is a polypeptide comprising a beta-pleated sheet region at an N terminus of a Telethonin molecule, or a homologue, an analogue or a derivative thereof.
Dependencies are inferred from the claim text.