Transluminal delivery of viruses for treatment of diseased tissue

A method for treating a disease or disorder in the body of a subject, said method comprising the steps of selecting a balloon catheter having two distally spaced-apart inflatable balloons coupled by an inflation lumen for simultaneous inflation or deflation thereof, and having an infusion lumen with at least one discharge port situated between the two balloons;

• advancing the balloon catheter transluminally into a vessel or duct of the body in direct fluid communication with affected tissue of the body containing defective cells attributable to or responsible for the disease or disorder to be treated, until a target site of the affected tissue is situated between the two balloons;
• inflating the two balloons through the inflation lumen while injecting through the infusion lumen of the catheter a body-compatible fluid containing a predetermined quantity of a virus selected for its ability to infect the defective cells so as to allay the defect therein while leaving normal cells unscathed, to discharge the quantity of virus in the fluid from the discharge port into a chamber at the target site formed by the space encompassed by the lining of the vessel or duct and the outer surface of the catheter between opposing surfaces of the two inflated balloons;
• and selectively increasing pressure of the discharged virus and fluid in the chamber to force the virus under an effective extravasation pressure through an endothelial barrier presented by the lining of the vessel or duct, without removing or otherwise injuring the endothelial barrier or the lining, for delivery of an effective dose of the virus into the defective cells of the affected tissue at the target site.

A method for treating a disease or disorder in the body of a subject, said method comprising the steps of selecting a balloon catheter with an inflatable balloon separated from the distal end and coupled to an inflation lumen of the catheter, and with an infusion lumen having a discharge port situated between the balloon and the distal end of the catheter, and a separate central lumen having an outlet port at the distal end of the catheter;

• advancing the balloon catheter transluminally into a vessel or duct of the body in direct fluid communication with affected tissue of the body containing defective cells attributable to or responsible for the disease or disorder to be treated, until the balloon is positioned such that a target site of the affected tissue lies between the balloon and the distal end of the catheter;
• inflating the balloon through the inflation lumen and dispensing body-compatible soluble beads through the central lumen for ejection from the outlet port, and injecting through the infusion lumen of the catheter a body-compatible fluid containing a predetermined quantity of a virus selected for its ability to infect the defective cells so as to allay the defect therein while leaving normal cells unscathed, to discharge the quantity of virus in the fluid from the discharge port into a chamber at the target site formed by the space encompassed by the lining of the vessel or duct and the outer surface of the catheter between the surface of the inflated balloon and the surface presented by a wall formed by the beads ejected from the distal end;
• and selectively increasing pressure of the discharged virus and fluid in the chamber by increasing the volume thereof to force the virus under an effective extravasation pressure through a barrier presented by the endothelial lining of the vessel or duct, without removing or otherwise injuring the endothelial lining, for delivery of an effective dose of the virus into the defective cells of the affected tissue at the target site.