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US 2017/0360850 A1Published application
Probiotic organisms for diagnosis, monitoring, and treatment of inflammatory bowel disease
AssigneesMassachusetts Institute of Technology, Synlogic, Inc.
InventorsTimothy Kuan-Ta Lu, Jacob Rosenblum Rubens, Isaak Elis Mueller, Gianluca Selvaggio, Paul Miller, Dean Falb, Vincent Isabella, Jonathan Kotula
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Abstract
Aspects of the present disclosure relate to genetically engineered organisms useful for the diagnosis and treatment of inflammatory bowel disease.
Claims26
1-110. (canceled)
A recombinant probiotic cell comprising:
- a sensor circuit, comprising:
- (a) a first promoter operably linked to a nucleic acid encoding a regulatory protein responsive to an input signal;
- and
- (b) a second promoter responsive to the regulatory protein and operably linked to a nucleic acid encoding a first output protein, wherein activity of the second promoter is altered when bound by the regulatory protein.
1 more element
A recombinant probiotic cell comprising:
- a sensor circuit, comprising:
- (a) a first promoter operably linked to a nucleic acid encoding a regulatory protein responsive to an input signal;
- (b) a second promoter responsive to the regulatory protein and operably linked to a nucleic acid encoding a first output protein, wherein activity of the second promoter is altered when bound by the regulatory protein;
- (c) an output molecule operably linked to a third promoter, wherein the output molecule or the third promoter is flanked by a first set of regulatory sequences, wherein the first set of regulatory sequences interacts with the first output protein to unlink the output molecule from the third promoter;
- (d) a fourth promoter responsive to the regulatory protein and operably linked to a nucleic acid encoding a second output protein, wherein activity of the fourth promoter is altered when bound by the regulatory protein;
- and, optionally,
- (e) a second output molecule flanked by a second set of regulatory sequences, wherein the second set of regulatory sequences interacts with the second output protein to operably link the second output molecule to a fifth promoter.
4 more elements
Dependencies are inferred from the claim text.