Compositions and methods for treating and preventing tissue injury and disease
AssigneeMicroVascular Tissues, Inc.
InventorsDale R. PETERSON, Ralph-Heiko Mattern, Corey Wilson-Wirth, Kevin L. Ohashi
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Abstract
The present invention provides novel compositions comprising multipotent cells or microvascular tissue, wherein the cells or tissue has been sterilized and/or treated to inactivated viruses, and related methods of using these compositions to treat or prevent tissue injury or disease in an allogeneic subject.
Claims7
A method of preparing a composition comprising isolated multipotent cells, wherein the composition has angiogenic or anti-inflammatory activity, the method comprising:
- (a) dissociating a tissue sample obtained from a donor mammal to release a plurality of multipotent cells therein;
- (b) separating a plurality of the released multipotent cells from one or more other tissue components to produce a composition comprising isolated multipotent cells;
- (c) optionally drying, lyophilizing, or cryopreserving the composition produced according to (b) or (d);
- and
- (d) sterilizing the composition resulting from (b) or (c) and/or inactivating virus present in composition resulting from (b) or (c),
- wherein the composition resulting from (d) retains measurable angiogenic or anti-inflammatory activity.
The method of claim 7, wherein:
- (i) the cells or tissue are not cultured;
- (ii) the method further comprises filtering the released cells or composition prior to (c);
- or
- (iii) the dissociation of (a) comprises contacting the tissue sample with one or more proteases, optionally when one or more proteases does not comprise collagenase.
A method of preparing a composition comprising processed microvascular tissue, wherein the composition has angiogenic or anti-inflammatory activity, the method comprising:
- (a) dissociating a microvascular tissue sample obtained from a donor mammal to produce a composition comprising dissociated microvascular tissue;
- (b) removing one or more tissue components from the composition produced according to (a);
- (c) optionally drying, lyophilizing, or cryopreserving the composition following (b) or (d);
- and
- (d) sterilizing the composition following (b) or (c) and/or inactivating virus present in the composition following (b) or (c);
- wherein the composition resulting from (c) or (d) retains measurable angiogenic or anti-inflammatory activity.
Dependencies are inferred from the claim text.