Enrichment Method of Ergosterol Peroxide from Sporoderm-Broken Ganoderma Lucidum Spore Powder

An enrichment method of Ergosterol peroxide from sporoderm-broken Ganoderma lucidum spore powder, comprising the following steps:

• (1) Preparing crude extract containing Ergosterol peroxide:
• taking the sporoderm-broken Ganoderma lucidum spore powder, performing extraction by percolation or reflux method, concentrating the extract liquid to dryness under reduced pressure, and obtaining the crude extract A containing Ergosterol peroxide;
• (2) Purification by medium pressure preparative chromatography:
• dissolving the crude extract A containing Ergosterol peroxide prepared in the step (1) with 60-95% methanol aqueous solution to obtain the sample solution for medium pressure preparative chromatography, performing purification by medium pressure preparative chromatography, collecting the fraction containing Ergosterol peroxide, concentrating it to dryness under reduced pressure to obtain the extractum B containing Ergosterol peroxide;
• (3) Purification by simulated moving-bed:
• dissolving the extractum B containing Ergosterol peroxide prepared in the step (2) with 60-95% methanol aqueous solution to obtain the sample solution, injecting the sample solution into the simulated moving-bed chromatography separation system for further purification, concentrating the extract liquor containing Ergosterol peroxide to dryness under reduced pressure, and obtaining the dry extract C rich in Ergosterol peroxide;
• (4) Dissolving the dry extract C rich in Ergosterol peroxide prepared in the step (3) with ethyl acetate-cyclohexane under heating conditions, transferring it into a environment at a temperature of 0-8° C. for recrystallization, filtering and washing the precipitate to obtain Ergosterol peroxide with high purity.