Full-function artificial organ fitting body as well as preparation and culture methods thereof

A preparation method of a full-function artificial organ fitting body, comprising the following steps:

• a) designing a shape structure, a branch arterial system structure, a branch venous system structure, a branch nervous system structure and a cortex layer structure of the full-function artificial organ respectively by adopting a three-dimensional computer modeling method;
• b) preparing overall cortex layer molds with open upper ends and different diameters, and a three-dimensional skeleton structure of the branch arterial systems, the branch venous systems and the branch nervous systems by adopting the 3D printing or in-mold pouring technology;
• and respectively preparing a natural polymer aqueous solution containing the adult cells or containing a mixture of the growth factors and the stem cells, a natural polymer aqueous solution containing the stem cells and a natural polymer aqueous solution containing the endothelial cells, wherein a mass percentage of the natural polymer aqueous solution is 0.1-20%, and a density of the cells in the natural polymer aqueous solution is 1*102-7 cells per mL;
• c) putting the three-dimensional skeleton structure of at least one group of the branch arterial systems, one group of the branch venous systems and the branch nervous system into the overall cortex layer mold with a smallest diameter, pouring the natural polymer aqueous solution containing the adult cells or containing the mixture of the growth factors and the stem cells among the branch arterial systems, the branch venous systems, the branch nervous systems and the overall cortex layer mold to form a lower part of a differentiation area, and spraying or injecting the natural polymer solution containing the endothelial cells or a mixture of the stem cells and the growth factors on the structure to form the docking area;
• d) putting a group of branch venous systems and branch nervous systems on an upper part of the docking area, or placing the branch arterial systems, the branch venous systems and the branch nervous systems, forming an upper part of a differentiation area from an opening at an upper end of the overall cortex layer mold according to a method in the step 3), and removing the overall cortex layer mold with the smallest diameter;
• e) sleeving a second overall cortex layer mold with a larger diameter on an outer part of the prepared differentiation area, pouring the natural polymer solution containing the stem cells into a gap, and crosslinking or polymerizing natural polymers to form a main body three-dimensional skeleton structure containing the differentiation area and the growth area;
• and
• f) removing the second overall cortex layer mold, coating a synthetic polymer solution layer outside the main body three-dimensional skeleton structure containing the differentiation area and the growth area, and extracting with an organic solvent to form the full-function organ fitting body containing a cortex layer structure.

A culture apparatus of a full-function artificial organ fitting body, comprising at least one inner airbag, an outer airbag, an inner airbag gas delivery apparatus (2), an outer airbag gas delivery apparatus (3), a sealing structure (6), an organ fitting body (8), a culture bottle (9), a fluid delivery apparatus (10) and an airbag controller (1), wherein the inner airbag gas delivery apparatus (2) and the outer airbag gas delivery apparatus (3) are respectively connected with the inner airbags and the outer airbag through gas pipelines;

• the inner airbags are put into the organ fitting body (8);
• the organ fitting body (8) is put into the outer airbag (4);
• the culture bottle (9) is filled with a culture solution;
• the culture solution is communicated with the organ fitting body through a fluid pipeline;
• the sealing structure (6) is arranged at inlets of the inner airbags and the outer airbag (4);
• and the airbag controller (1) is respectively connected with the inner airbag gas delivery apparatus (2) and the outer airbag gas delivery apparatus (3) through a control circuit.