Back to results
US 2017/0356014 A1Published application
Multi-enzyme conjugate, method for preparing the same and method for preparing organic compound using the same
AssigneeGwangju Institute of Science and Technology
InventorsInchan KWON, Jinhwan CHO, Sung In LIM
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to a multi-enzyme conjugate, a method for preparing the same and a method for preparing an organic compound using the same. More particularly, a multi-enzyme conjugate exhibiting improved catalytic efficiency over respective free enzymes using site-specific incorporation of a clickable non-natural amino acid into the enzymes and two compatible click reactions, a method for preparing the same and a method for preparing an organic compound using the same may be provided.
Claims20
A multi-enzyme conjugate comprising a conjugate of a first enzyme-linker and a second enzyme-linker, wherein
- the first enzyme-linker is a conjugate of a first modified enzyme and a first linker,
- the first modified enzyme comprises (i) a first enzyme and (ii) one or more first non-natural amino acid (NNAA) comprising a click functional group 1-1 and the first non-natural amino acid is site-specifically incorporated into a first enzyme residue of the first enzyme,
- the first linker comprises a click functional group 1-2 and a click functional group 2-1,
- the first enzyme-linker is a conjugate formed from a first click reaction of the click functional group 1-1 of the first modified enzyme and the click functional group 1-2 of the first linker,
- the second enzyme-linker is a conjugate of a second modified enzyme and a second linker,
- the second linker comprises a click functional group 2-2 and a click functional group 3-1,
- the second modified enzyme comprises (i) a second enzyme and (ii) one or more second non-natural amino acid comprising a click functional group 3-2 and the second non-natural amino acid is site-specifically incorporated into a second enzyme residue of the second enzyme,
- the second enzyme-linker is a conjugate formed from a third click reaction of the click functional group 3-1 of the second linker and the click functional group 3-2 of the second modified enzyme, and
- the conjugate of the first enzyme-linker and the second enzyme-linker is a conjugate formed from a second click reaction of the click functional group 2-1 of the first linker and the click functional group 2-2 of the second linker.
6 more elements
A method for preparing a multi-enzyme conjugate, comprising
- (C) a step of coupling a first enzyme-linker with a second enzyme-linker, wherein
- the first enzyme-linker is a conjugate of a first modified enzyme and a first linker,
- the first modified enzyme comprises (i) a first enzyme and (ii) one or more first non-natural amino acid (NNAA) comprising a click functional group 1-1 and the first non-natural amino acid is site-specifically incorporated into a first enzyme residue of the first enzyme,
- the first linker comprises a click functional group 1-2 and a click functional group 2-1,
- the first enzyme-linker is a conjugate formed from a first click reaction of the click functional group 1-1 of the first modified enzyme and the click functional group 1-2 of the first linker,
- the second enzyme-linker is a conjugate of a second modified enzyme and a second linker,
- the second linker comprises a click functional group 2-2 and a click functional group 3-1,
- the second modified enzyme comprises (i) a second enzyme and (ii) one or more second non-natural amino acid comprising a click functional group 3-2 and the second non-natural amino acid is site-specifically incorporated into a second enzyme residue of the second enzyme,
- the second enzyme-linker is a conjugate formed from a third click reaction of the click functional group 3-1 of the second linker and the click functional group 3-2 of the second modified enzyme, and
- the step (C) is performed by a second click reaction of the click functional group 2-1 of the first linker and the click functional group 2-2 of the second linker.
7 more elements
A method for preparing a multi-enzyme conjugate, comprising
- (B1) a step of obtaining a first enzyme-linker by coupling a first modified enzyme with a first linker,
- (B2) a step of obtaining a second enzyme-linker by coupling a second modified enzyme with a second linker, and
- (C) a step of coupling the first enzyme-linker with the second enzyme-linker, wherein
- the first modified enzyme comprises (i) a first enzyme and (ii) one or more first non-natural amino acid (NNAA) comprising a click functional group 1-1 and the first non-natural amino acid is site-specifically incorporated into a first enzyme residue of the first enzyme,
- the first linker comprises a click functional group 1-2 and a click functional group 2-1,
- the step (B1) is performed by a first click reaction of the click functional group 1-1 of the first modified enzyme and the click functional group 1-2 of the first linker,
- the second linker comprises a click functional group 2-2 and a click functional group 3-1,
- the second modified enzyme comprises (i) a second enzyme and (ii) one or more second non-natural amino acid comprising a click functional group 3-2 and the second non-natural amino acid is site-specifically incorporated into a second enzyme residue of the second enzyme,
- the step (B2) is performed by a third click reaction of the click functional group 3-1 of the second linker and the click functional group 3-2 of the second modified enzyme, and
- the step (C) is performed by a second click reaction of the click functional group 2-1 of the first linker and the click functional group 2-2 of the second linker.
7 more elements
Dependencies are inferred from the claim text.