Universal method for functionalization of dyed microspheres

Abstract

Provided are processes of functionalizing a microparticle that include reacting a microparticle expressing a carboxylic acid with a functionalization linker including the structure N-L1-A where N is a free amine, L1 is a linker, and A is an azide and an alkyne terminated group, to form a functional group terminated microparticle, and forming a functionalized microparticle by reacting the functional group terminated microparticle with a peptide including a terminal functional group comprising an alkyne or azide, where the peptide includes the structure F-L2-Peptide, where F is a functional group and L2 is a linker. Also provided are compositions suitable for effectively coupling a peptide to a microsphere and functionalized microspheres suitable for reacting with a detection agent.

Dependencies are inferred from the claim text.