Pestivirus
AssigneeIntervet Inc.
InventorsAd de Groof, Lars Guelen, Carla Christina Schrier, Martin Deijs, Cornelia Maria van der Hoek
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Abstract
The present invention relates to a novel porcine pestivirus, to proteins of the virus and to vaccines based upon the virus and proteins thereof. The invention also relates to DNA fragments comprising a gene of the virus and to DNA vaccines based upon genes of the virus. Furthermore the invention relates to antibodies that are reactive with the novel virus and to diagnostic tests for the detection of the virus or antibodies against the virus.
Claims29
An isolated virus which is a member of the pestiviruses, wherein said virus:
- a) is the causative agent of Group A-II congenital tremors in pigs and
- b) has a viral genome comprising a gene encoding an envelope protein Erns, a gene encoding an envelope protein E2 and a gene encoding an envelope protein E1, wherein the nucleotide sequence of the Erns gene has a level of identity of at least 80% to the nucleotide sequence of SEQ ID NO:
- 1 and/or the nucleotide sequence of the E2 gene has a level of identity of at least 80% to the nucleotide sequence of SEQ ID NO:
- 3 and/or the nucleotide sequence of the E1 gene has a level of identity of at least 80% to the nucleotide sequence of SEQ ID NO:
- 5.
An isolated virus which is a member of the pestiviruses, wherein said virus:
- a) is the causative agent of Group A-II congenital tremors in pigs and
- b) the cDNA reverse-transcribed from the viral RNA genome reacts in a PCR reaction with a primer set of SEQ ID NO:
- 7 and 8 to give a PCR product of 156+/−10 base pairs and/or reacts in a PCR reaction with a primer set as depicted in SEQ ID NO:
- 9 and 10 to give a PCR product of 335+/−10 base pairs and/or reacts in a PCR reaction with a primer set of SEQ ID NO:
- 11 and 12 to give a PCR product of 896+/−10 base pairs and/or reacts in a PCR reaction with a primer set of SEQ ID NO:
- 13 and 14 to give a PCR product of 896+/−10 base pairs and/or reacts in a PCR reaction with a primer set of SEQ ID NO:
- 15 and 16 to give a PCR product of 182+/−10 base pairs and/or reacts in a PCR reaction with a primer set of SEQ ID NO:
- 17 and 18 to give a PCR product of 182+/−10 base pairs.
A gene encoding an Erns protein, wherein the nucleotide sequence of said gene has a level of identity of at least 80% to the nucleotide sequence of SEQ ID NO:
- 1.
A gene encoding an E2 protein, wherein the nucleotide sequence of said gene has a level of identity of at least 80% to the nucleotide sequence of SEQ ID NO:
- 3.
A gene encoding an E1 protein, wherein the nucleotide sequence of said gene has a level of identity of at least 80% to the nucleotide sequence of SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.