Closed-system culture vessel, transport method, and automated culturing device

The closed system culture vessel for holding cells comprising:

• a first semi-open space;
• a second semi-open space arranged in order to surround the first semi-open space;
• and a lid portion for sealing the first semi-open space,
• wherein the lid portion forms a closed space with the first semi-open space,
• the lid portion is in contact with the opening of the second semi-open space over the entire circumference,
• transferring of the liquid held within the second semi-open space into the first semi-open space surrounding the second semi-open space is controlled by the contact between the lid portion and the opening of the second semi-open space, and makes it possible to hold the liquid in the second semi-open space.

An automated culture apparatus for culturing cells, comprising:

• a holding part for holding a liquid or a gas required for culturing;
• a closed system culture vessel for holding the liquid or the gas supplied from the holding part;
• a passage part provided with a plurality of passages for connecting the holding part with the closed system culture vessel;
• a control unit for controlling the passage part, and for controlling so as to supply or discharge the liquid or the gas from the holding part to the closed system culture vessel,
• wherein the closed system culture vessel is provided with a first vessel, a second vessel arranged within the first vessel and having a porous membrane coupling the opening with the first vessel side, and a lid portion for sealing the first vessel,
• the lid portion has a connector part for performing the supply or discharge of the liquid or the gas to the first vessel and the second vessel, and
• the lid portion forms a closed space with the first vessel, and the lid portion is in contact with the opening of the second vessel over the entire circumference, controlling transfer of the liquid supplied and held within the second vessel to the first vessel to make it possible to hold the liquid in the second vessel.