Illumination setting method, light sheet microscope apparatus, and recording medium
AssigneeOlympus Corporation
InventorYoshihito IGUCHI
Life of this patent
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Abstract
An illumination setting method includes acquiring an image of a sample onto which a light sheet has been radiated; determining, on the basis of the acquired image of the sample, a subordinate ray angle with respect to a width direction of the light sheet; and performing a setting of the illumination optical system according to the determined subordinate ray angle.
Claims19
An illumination setting method comprising:
- acquiring an image of a sample onto which a light sheet emitted from an illumination optical system has been radiated;
- determining, on the basis of the acquired image of the sample, a subordinate ray angle with respect to a width direction of the light sheet emitted from the illumination optical system;
- and
- performing a setting of the illumination optical system according to the determined subordinate ray angle.
An illumination setting method comprising:
- acquiring an image of a sample onto which a light sheet has been radiated by an illumination optical system;
- determining, on the basis of the acquired image of the sample, an incident angle at which a principal ray of the light sheet emitted from the illumination optical system enters the sample;
- and
- performing at least one of a setting of the illumination optical system and a setting of a direction of the sample according to the determined incident angle.
An illumination setting method comprising:
- acquiring, by a computer and from an imaging device, an image of a sample onto which a light sheet has been radiated by an illumination optical system;
- determining, by the computer and on the basis of the image of the sample that has been acquired from the imaging device, a subordinate ray angle with respect to a width direction of the light sheet emitted from the illumination optical system;
- and
- outputting, by the computer, a control signal that gives an instruction to perform a setting of the illumination optical system that corresponds to the determined subordinate ray angle.
A light sheet microscope apparatus comprising:
- an illumination optical system that radiates a light sheet onto a sample;
- an imaging device that acquires an image of the sample onto which the light sheet has been radiated by the illumination optical system;
- a controller that determines, on the basis of the image of the sample that has been acquired by the imaging device, a subordinate ray angle with respect to a width direction of the light sheet emitted from the illumination optical system;
- and
- a setting device that performs a setting of the illumination optical system according to the subordinate ray angle determined by the controller.
A non-transitory recording medium having stored therein a program that causes a computer to execute a process comprising:
- acquiring, from an imaging device, an image of a sample onto which a light sheet has been radiated by an illumination optical system;
- determining, on the basis of the image of the sample that has been acquired from the imaging device, a subordinate ray angle with respect to a width direction of the light sheet emitted from the illumination optical system;
- and
- outputting a control signal that gives an instruction to perform a setting of the illumination optical system that corresponds to the determined subordinate ray angle.
Dependencies are inferred from the claim text.