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US 2017/0322403 A1Published application
Multi-focal structured illumination microscopy systems and methods
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Serv
InventorsHari Shroff, Andrew York
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Abstract
Various embodiments for a multi-focal selective illumination microscopy (SIM) system for generating multi-focal patterns of a sample are disclosed. The multi-focal SIM system performs a focusing, scaling and summing operation on each generated multi-focal pattern in a sequence of multi-focal patterns that completely scan the sample to produce a high resolution composite image.
Claims13
A microscopy system comprising:
- a light source for transmitting a single light beam;
- a first microlens array for splitting the single light beam into a plurality of light beams for forming at least one multi-focal pattern;
- a scanner for scanning the plurality of light beams that forms the at least one multi-focal pattern onto a sample such that the sample generates a plurality of fluorescent emissions with each of the at least one multi-focal pattern;
- a pinhole array to block out-of-focus fluorescent emissions for each of the at least one multi-focal pattern and allowing through in-focus florescent emissions to pass through the pinhole array;
- a second microlens array for producing a non-inverted image of the plurality of light beams having a one half magnification, wherein the scanner rescans the non-inverted image of the plurality of light beams;
- and
- a camera for capturing the scanned non-inverted image.
4 more elements
A microscopy system comprising:
- a light source for transmitting a single light beam;
- a first spinning disk with converging microlens for rotation in a first direction and positioned along an optic axis for splitting the single light beam into a plurality of light beams forming a multi-focal pattern;
- a second spinning disk with a pinhole array for rotation in the first direction and positioned along the optic axis for blocking out-of-focus light beams of the plurality of light beams for each multi-focal pattern and allowing through in-focus light beams of the plurality of light beams to pass through the spinning disk with a pinhole array, and
- a third spinning disk with a diverging microlens for rotation in the first direction and positioned along the optic axis, wherein the first spinning disk with converging microlens, the second spinning disk with a pinhole array, and the third spinning disk with a diverging microlens rotate in sync relative to each other.
1 more element
Dependencies are inferred from the claim text.